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Gastric cancer is the most common cancer and the second leading cause of cancer-related death worldwide. Human gastric cancers with high expression of DNA methyltransferase (DNMT) have a worse prognosis than those with low DNMT expression. We investigated the effect of decitabine (5-aza-2’deoxycytidine, DAC), a hypomethylating agent, on xenograft growth and on the clinicopathologic characteristics in human gastric cancer. Sodium butyrate (NaBu), an HDAC inhibitor, was also used. SW-480 cells and MKN-45 cells were treated with DAC at a dose of 2.5-8.0 micromol/L for 72 h and with DAC at 2.5-8.0 micromol/L and NaBu at 1.0-5.0 mmol/L for 72 h. An implantation model of human gastric cancer was established in athymic nude mice. These cells were injected into the mice and tumor growth was evaluated. Cells treated with DAC had a suppressive effect on the growth of gastric cancer cells in vitro. Treatment of mice with DAC and NaBu suppressed the growth of gastric cancer. Apoptosis of tumors occurred in these mice. The tumor cells in the liver of mice treated with DAC and NaBu were decreased in number. The DNMT1, DNMT3A, and DNMT3B were downregulated by treatment with DAC or by NaBu. Human gastric cancer with high DNMT expression can be suppressed by treatment with DAC. Both DAC and NaBu induce apoptosis of tumors and have a preventive effect on the growth of gastric cancer cells.static inline void
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